Customization: | Available |
---|---|
Polishing: | Polishing |
Thickness: | 1mm |
Still deciding? Get samples of US$ 1/Piece
Request Sample
|
Suppliers with verified business licenses
Audited by an independent third-party inspection agency
Microscope slide is a transparent glass sheet used in biological experiments, with a length and width of approximately 75 mm and 26 mm, and a thickness of 1 mm. It is used as a carrier sample for observation under a microscope. The sample will be fixed on a glass slide and moved together under a microscope for observation. The size of the glass slide allows multiple samples to be viewed sequentially under the microscope, removed from the microscope, labeled, transported, and stored in an appropriate manner.
A glass slide is often paired with another smaller and thinner transparent glass sheet, called a cover glass. Place the cover glass on the sample and slide.
Usage:
1. Smearing method is a method of preparing slides by uniformly coating materials onto them.
Smear materials include single-cell organisms, small algae, blood, bacterial culture media, loose tissues of animals and plants, testes, anthers, etc.
When smearing, attention should be paid to: (1) The glass slide must be clean. (2) The glass slide should be flat. (3) The coating must be uniform. Apply the droplet to the middle right of the glass slide and spread it evenly with a dissecting blade or toothpick. (4) The coating should be thin. Using another glass slide as a slide, gently push from right to left along the surface of the glass slide with the applied liquid (the angle between the two slides should be 30 ° -45 °), and apply a uniform thin layer. (5) Fixed. If fixation is required, chemical fixatives or drying methods (bacteria) can be used for fixation. (6) Staining. Bacteria use methylene blue, blood uses Wright's staining solution, and sometimes iodine can be used. The staining solution should cover the entire coating surface. (7) Rinse. Use absorbent paper to absorb or dry. (8) Seal the film. Seal with Canadian gum for long-term preservation.
2. The compression method is a method of slicing biological materials by placing them between a glass slide and a cover, applying a certain pressure to disperse tissue cells.
3. The mounting method is a method of making slide specimens by encapsulating biological materials as a whole, which can be used to make temporary or permanent mounting.
The packaging materials include: microorganisms such as algae, sponges, amoebas, and nematodes; Hydra, the leaf epidermis of plants; Insect wings, feet, mouthparts, human oral epithelial cells, etc.
When using the mounting method, attention should be paid to: (1) When holding the glass slide, it should be kept flat or placed on a platform. When dripping water, the amount of water should be appropriate, just enough to cover the glass slide. (2) The material should be unfolded with a dissecting needle or forceps to avoid overlap and flattened on the same plane. (3) When placing the cover glass, slowly cover it on the water droplet from one side to prevent the formation of bubbles. (4) When staining, place a drop of staining solution on one side of the cover glass and use absorbent paper to attract it from the other side, so that the specimen under the cover glass is uniformly stained. After coloring, use the same method to drop a drop of water, aspirate the staining solution, and observe under a microscope.
4. Slices are glass slide specimens made from thin slices cut from living organisms.
Due to different requirements, manual sectioning can be performed using a blade, or tissue blocks can be embedded in paraffin or colchicine or frozen at low temperatures and sliced using a microtome. Cut into 5-10 micron thin slices for observation under an optical microscope. Ultra thin sections made by embedding tissue blocks with epoxy resin or methyl acrylic acid, with a thickness of 20-50 nanometers, specifically designed for observation under an electron microscope. Slices used for general teaching purposes, such as root tips and stems, are commonly referred to as paraffin sections
Customized support | OEM, ODM, OBM |
Size | 25x75mm |
Material | GLASS |
Application | histology |