| Customization: | Available |
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| Certification: | CE, ISO, RoHS |
| Material: | Rubber / Plastic |
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PCR tubes are essential laboratory consumables used in the polymerase chain reaction (PCR) process, a technique widely employed in molecular biology to amplify DNA sequences. These tubes are typically made of high-quality polypropylene, which ensures durability and resistance to high temperatures required during the PCR cycles. The most common size for PCR tubes is 0.2 mL, although other sizes such as 0.1 mL and 0.5 mL are also available.
Key Features:
1.
Material: Made from high-grade polypropylene, PCR tubes are designed to withstand the thermal cycling conditions of PCR, including repeated heating and cooling cycles.
2.
Thin-Walled Design: The thin walls of PCR tubes ensure efficient and uniform heat transfer, which is crucial for the accuracy and efficiency of the PCR process.
3.
Cap Types: PCR tubes come with various cap designs, including flat caps, domed caps, and attached caps. Flat caps are commonly used as they provide a stable base and prevent sample evaporation.
4.
Sterility: Many PCR tubes are available in sterile formats, free from DNase, RNase, and human genomic DNA, to prevent contamination and ensure the integrity of the samples.
5.
Compatibility: PCR tubes are compatible with most standard thermal cyclers, making them versatile for various laboratory setups.
6.
Volume: The standard volume for PCR tubes is 0.2 mL, but they are also available in other volumes to accommodate different experimental needs.
7.
Color Options: PCR tubes are available in different colors, including transparent and white. White tubes are particularly useful for qPCR as they enhance fluorescence signal detection.
8.
Sealing: The caps of PCR tubes are designed to provide a tight seal, preventing sample evaporation and contamination during the PCR process.
9.
Ease of Use: PCR tubes are designed for easy handling and can be used individually or in strips, depending on the experimental requirements.
10.
Applications: PCR tubes are used in various applications, including DNA amplification, cloning, sequencing, and diagnostic testing.
Applications:
PCR tubes are widely used in molecular biology, clinical diagnostics, and research laboratories. They are essential for:
• DNA Amplification: PCR tubes are used to amplify specific DNA sequences, making them detectable and analyzable.
• Cloning: PCR tubes facilitate the cloning of DNA fragments for further genetic analysis and manipulation.
• Sequencing: PCR tubes are used in the preparation of DNA samples for sequencing, allowing researchers to determine the nucleotide sequence of DNA.
• Diagnostic Testing: PCR tubes are crucial in diagnostic tests for detecting genetic disorders, infectious diseases, and other medical conditions.
• Research: PCR tubes are used in various research applications, including gene expression studies, mutation analysis, and genotyping.
Usage Instructions:
1.
Sample Preparation: Collect the DNA sample and prepare the PCR reaction mixture, including primers, polymerase enzyme, and other reagents.
2.
Loading: Dispense the reaction mixture into the PCR tubes, ensuring the correct volume is used.
3.
Sealing: Securely close the tubes with the appropriate caps to prevent evaporation and contamination.
4.
Thermal Cycling: Place the tubes in the thermal cycler and run the PCR program, which includes denaturation, annealing, and extension steps.
5.
Analysis: After the PCR process is complete, analyze the amplified DNA using appropriate techniques such as gel electrophoresis or qPCR.
Maintenance and Storage:
• Store PCR tubes in a cool, dry place away from direct sunlight to maintain their integrity.
• Ensure that the tubes are properly sealed and stored in their original packaging to prevent contamination.
• Regularly check the tubes for any signs of damage or contamination before use.
Quality Assurance:
PCR tubes are manufactured under strict quality control standards to ensure their reliability and performance. They are tested for sterility, DNase/RNase-free status, and compatibility with standard thermal cyclers.
| Color | Transparent |
| Volume | 0.2ML |
| Materia | pp |
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